US President Barack Obama
US President Barack Obama has said the use of chemical weapons by Syria would be a "red line" that would change his thinking on intervention in the crisis, reports the BBC.
He said he had "at this point not ordered military engagement".
But he added: "There would be enormous consequences if we start seeing movement on the chemical weapons front or the use of chemical weapons."
Earlier the new UN special envoy to Syria faced criticism for refusing to say whether President Assad must quit.
President Obama, speaking to reporters at a White House briefing, said the deployment or use of biological weapons would widen the conflict in the region.
He said: "It doesn't just include Syria. It would concern allies in the region, including Israel, and it would concern us."
He warned President Bashar al-Assad and "other players on the ground" about the use or movement of such weapons.
He said: "A red line for us is [if] we see a whole bunch of chemical weapons moving around, or being utilised. That would change my calculus."
Syria holds the world's fourth-largest stockpile of chemical weapons. Last month a Syrian foreign ministry spokesman said the weapons would never be deployed inside Syria.
However, the BBC's Kim Ghattas in Washington says the US has seen unconfirmed reports recently that the Syrian authorities have been moving the country's chemical arms stockpile.
Earlier on Monday, the UN's new envoy to Syria, Lakhdar Brahimi told the BBC that he was "not in a position to say yet" whether President Assad should go, but was "committed to finding a solution".
Brahimi, a former Algerian foreign minister, last week succeeded Kofi Annan who resigned after both sides largely ignored his peace plan.
On Sunday, UN observers ended their mission to verify its implementation.
Their departure came after the UN Security Council agreed to allow their mandate to expire at midnight, and instead set up a new civilian office in Damascus to pursue political contacts that might lead to peace.
Since being confirmed as the new UN and Arab League envoy to Syria, Brahimi has acknowledged that he has no concrete ideas of how to end the conflict, which he believes has been a civil war for some time.
On Monday, he told the BBC that he was not ready to say whether President Assad should step down despite widespread international condemnation of his government's crackdown on dissent since protests erupted in March 2011.